Effect of phorbol and Bryostatin I on chondrogenic expression of chick limb bud, in vitro

Journal Article · Mon Oct 26 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90480-2· OSTI ID:6062388

Garrison, J C; Pettit, G R; Uyeki, E M

The present paper describes the effects of PMA (phorbol 12-myristate 13 acetate) on in vitro chondrogenesis in non-passaged, embryonic limb bud cells, relative to the effects of Bryostatin I. This compound also activates C kinase and binds competitively to the phorbol ester receptor, yet does not affect cell differentiation. Levels of PMA as low as 10/sup -7/ M markedly reduced cartilage formation in 4-day cultures, as indicated by nodule count and Alcian blue staining for chondroitin sulfate. Coadministration of Bryostatin I at equimolar concentration prevented the PMA inhibitory effect on chondrocytic expression. This confirms other findings that phorbol activation of C kinase cannot exclusively account for the activity of phorbol on cell expression. Altering the time of PMA exposure demonstrated that PMA inhibited chondrocyte phenotypic expression, rather than cell commitment: early exposure to PMA had little inhibitor effect on the staining index, whereas, exposure from 49-96 h and 0-96 h had moderate and strong inhibitory effects, respectively, on cartilage synthesis. Further research on the phorbol/Bryostatin I interaction should add to their knowledge of the control processes involved in tumor promotion and cell differentiation. 21 references, 3 figures.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 6062388
• Journal Information: Life Sci.; (United States), Vol. 41:17
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
EMBRYONIC CELLS
CELL DIFFERENTIATION
PHORBOL ESTERS
BIOLOGICAL PATHWAYS
CHICKENS
EXPERIMENTAL DATA
IN VITRO
INHIBITION
ONTOGENESIS
PHOSPHOTRANSFERASES
ANIMAL CELLS
ANIMALS
BIRDS
CARCINOGENS
DATA
DRUGS
ENZYMES
ESTERS
FOWL
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology