Linkage mapping of the human CSF2 and IL3 genes

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.11.4821· OSTI ID:6056581

Frolova, E I; Dolganov, G M; Mazo, I A; Smirnov, D V ^[1]; Copeland, P; Stewart, C; Dean, M ^[2]; O'Brien, S J ^[3]

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1. M.M. Shemyakin Inst. of Bio-organic Chemistry, Moscow (USSR)
2. Program Resources, Inc./DynCorp., Research Triangle Park, NC (United States)
3. National Cancer Inst., Frederick, MD (United States)

Interleukin 3 (encoded by the IL3 gene) and granulocyte-macrophage colony-stimulating factor (encoded by the CSF2 gene) are small secreted polypeptides that bind to specific cell surface receptors and regulate the growth, gene expression, and differentiation of many of the hematopoietic cell lineages, particularly nonlymphoid cells. The IL3 and CSF2 genes have been cloned and mapped to human chromosome bands 5q23-31. Only 10 kilobases of dna separates the two genes, suggesting that they have a common origin and/or regulation. The authors have cloned 70 kilobases of genomic DNA that includes the IL3 and CSF2 genes, as well as flanking sequences, and report a physical map of this region. Several unique-sequence DNA segments have been identified in this region, and one of these fragments detects two restriction fragment length polymorphisms in DNA from unrelated Caucasians. Segregation of these DNA polymorphisms was followed in the Centre Etude du Polymorphisme Humaine (CEPH) panel of 40 large three-generation pedigrees, and linkage was detected with 17 genetic markers previously typed in these families. Multipoint linkage analysis permits the placement of the region containing the IL3 and CSF2 structural genes on the recombination-genetic linkage map of chromosome 5q and thereby allows the role of these genes in leukemogenesis to be more critically examined.

• OSTI ID: 6056581
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:11; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA-CLONING
HUMAN CHROMOSOME 5
GENETIC MAPPING
CARCINOGENESIS
GENE RECOMBINATION
LYMPHOKINES
OLIGONUCLEOTIDES
RFLPS
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
GROWTH FACTORS
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
550201* - Biochemistry- Tracer Techniques