Decreased arachidonic acid content and metabolism in tissues of NZB/W F1 females fed a diet containing 0. 45% dehydroisoandrosterone (DHA)
A diet containing 0.45% DHA fed to NZB/W mice, a model of systemic lupus erythematosus, delays the time of onset, improves survival and decreases the formation of antibodies to ds-DNA. Essential fatty acid-deficient diets or inclusion of eicosapentaenoic acid have similar beneficial effects and led them to investigate arachidonic acid metabolism in response to feeding DHA. The arachidonic acid content of plasma cholesteryl ester decreased from 37.4 +/- 2.2 to 28.2 +/- 1.3 mg%. In total liver phospholipid the value decreased from 18.1 +/- 0.52 to 13.7 +/- 1.3 mg%, in total kidney phospholipid the value decreased from 24.10 +/- 0.87 to 20.7 +/- 0.32 mg% and in resident peritoneal macrophages the value decreased from 15.4 +/- 4.6 to 3.6 +/- 1.4 mg%. The metabolism of exogenous (1-/sup 14/C)arachidonic acid by resident peritoneal macrophages in response to Zymosan stimulation for 2 hr was examined by extraction of metabolites and separation by HPLC. Cells isolated from DHA-fed animals produced less PGE2 than controls, yet similar amounts of 6-keto PGF1..cap alpha.. were produced. Arachidonic acid metabolites have significant effects on the immune system and may be a mechanism involved in the benefits obtained by inclusion of DHA in the diet.
- Research Organization:
- Univ. of Texas Health Science Center, Dallas
- OSTI ID:
- 6026737
- Report Number(s):
- CONF-870644-; TRN: 87-037303
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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ANDROSTERONE
BIOCHEMICAL REACTION KINETICS
ARACHIDONIC ACID
METABOLISM
SKIN DISEASES
PATHOGENESIS
BIOLOGICAL MODELS
CARBON 14 COMPOUNDS
DIET
LIQUID COLUMN CHROMATOGRAPHY
LIVER
METABOLITES
TRACER TECHNIQUES
ANDROGENS
ANDROSTANES
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISEASES
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques