Radiosensitivity of kidney colony-forming cells: a short-term assay in situ in the mouse
A short-term colony assay for renal tubule epithelium has been developed. Uranyl nitrate (UN) is a heavy metal nephrotoxin that induces acute tubule necrosis followed by a large compensatory increase in the rate of cell proliferation in the nephron. UN was used to precipitate latent damage following renal irradiation. Using a subcapsular colony count at 14 days after unilateral irradiation, a single-dose cell survival curve was obtained with a D0 of 4.2 +/- 0.3 Gy. High-dose irradiation of an exteriorized kidney resulted in a survival curve which was biphasic, with a plateau in survival between 18 and 40 Gy. Subtraction of this plateau level from all the survival data gave D0 values of 2.5 +/- 0.2 Gy (data analyzed between 7.5 and 16 Gy) or 2.0 +/- 0.2 Gy (over range 12-16 Gy). The D0 value obtained at 20 months after bilateral (or unilateral) kidney irradiation, without the use of UN, was 2.9 +/- 1.1 Gy (over range 10-14 Gy).
- Research Organization:
- Paterson Institute for Cancer Research, Manchester (England)
- OSTI ID:
- 6024117
- Journal Information:
- Radiat. Res.; (United States), Vol. 118:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NITRATES
TOXICITY
STEM CELLS
RADIOSENSITIVITY
BIOASSAY
CELL PROLIFERATION
COLONY FORMATION
EPITHELIUM
KIDNEYS
MICE
NECROSIS
SURVIVAL CURVES
URANYL COMPOUNDS
ACTINIDE COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
MAMMALS
NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RODENTS
SOMATIC CELLS
TISSUES
URANIUM COMPOUNDS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology