Role of cell surface carbohydrate on herpes virus type I (HSV-1) entry
The role of cell surface glycopeptides (GP) or oligosaccharides (OS) on HSV-1 (strain HF) attachment was studied by isolating those macromolecules by proteolytic digestion of uninfected cells and/or hydrazinolysis of whole cells or plasma membrane fractions. Variable amounts of GP or OS were mixed with 100-200 pfu of (/sup 3/H)-HSV incubated for 45 min at 20/sup 0/C, and infectivity determined. GP were fractionated by lectin affinity-, ion exchange- and molecular sieve column chromatography. A GP fraction containing 13 ..mu..g reducing sugar/ml inhibited plaque formation by 80%. sigma-elimination or the removal of NeuNAc had no affect. OS obtained by hydrazinolysis, inhibited plaque formation by 86-100% at concentrations of 14-20 ..mu..g reducing sugars/ml. Only complex OS, obtained by treating delipidated, whose cells with endoglycosidase D, blocked attachment; whereas high mannose fractions had no effect. These OS failed to inhibit fusion suggesting that N-linked complex OS are part of the receptor site(s).
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6024084
- Report Number(s):
- CONF-870644-; TRN: 87-034003
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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HERPES SIMPLEX
INFECTIVITY
OLIGOSACCHARIDES
BIOLOGICAL FUNCTIONS
PEPTIDES
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ION EXCHANGE CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CARBOHYDRATES
CHROMATOGRAPHY
DISEASES
FUNCTIONS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PROTEINS
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SEPARATION PROCESSES
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