Presentation of human minor histocompatibility antigens by HLA-B35 and HLA-B38 molecules

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.7.2583· OSTI ID:5989541

Yamamoto, Junji; Kariyone, Ai; Kano, Kyoichi; Takiguchi, Masafumi; Akiyama, Nobuo ^[1]

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1. Univ. of Tokyo (Japan)

Cytotoxic T lymphocyte (CTL) clones specific for human minor histocompatibility antigens (hmHAs) were produced from a patient who had been grafted with the kidneys from his mother and two HLA-identical sisters. Of eight CTL clones generated, four recognized an hmHA (hmHA-1) expressed on cells from the mother and sister 3 (second donor); two recognized another antigen (hmHA-2) on cells from the father, sister (third donor), and sister 3; and the remaining two clones recognized still another antigen (hmHA-3) on cells from the father and sister 3. Panel studies revealed that CTL recognition of hmHA-1 was restricted by HLA-B35 and that of hmHA-2 and hmHA-3 was restricted by HLA-B38. The HLA-B35 restriction of the hmHA-1 -specific CTL clones was substantiated by the fact that they killed HLA-A null/HLA-B null Hmy2CIR targets transfected with HLA-B35 but not HLA-B51, -Bw52, or -Bw53 transfected Hmy2CIR targets. These data demonstrated that the five amino acids substitutions on the {alpha}{sub 1} domain between HLA-B35 and -Bw53, which are associated with Bw4/Bw6 epitopes, play a critical role in the relationship of hmHA-1 to HLA-B35 molecules. The fact that the hmHA-1-specific CTLs failed to kill Hmy2CIR cells expressing HLA-B35/51 chimeric molecules composed of the {alpha}{sub 1} domain of HLA-B35 and other domains of HLA-B51 indicated that eight residues on the {alpha}{sub 2} domain also affect the interaction of hmHA-1 and the HLA-B35 molecules.

• OSTI ID: 5989541
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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CONNECTIVE TISSUE CELLS
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INTERMEDIATE MASS NUCLEI
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550201* - Biochemistry- Tracer Techniques