Chemical synthesis of high specific-activity (/sup 35/S)adenosylhomocysteine
The study of the family of transmethylases, critical to normal cellular function and often altered in cancer, can be facilitated by the availability of a high specific-activity S-adenosylhomocysteine. The authors report the two-step preparation of (/sup 35/S)adenosylhomocysteine from (/sup 35/S)methionine at a specific activity of 1420 Ci/mmol in an overall yield of 24% by a procedure involving demethylation of the (/sup 35/S)methionine to (/sup 35/S)homocysteine followed by condensation with 5'-chloro-5'-deoxyadenosine. The ease of the reactions, ready availability and low cost of the reagents and high specific-activity and stability of the product make the procedure an attractive one with many uses, and superior to current methodology.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 5952500
- Journal Information:
- Anal. Biochem.; (United States), Vol. 158:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LABELLED COMPOUNDS
CHEMICAL PREPARATION
ADENOSINE
HOMOCYSTEINE
METHIONINE
METHYLATION
SULFUR 35
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RIBOSIDES
SULFUR ISOTOPES
SYNTHESIS
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials