Gibberellin A sub 3 is biosynthesized from gibberellin A sub 20 via gibberellin A sub 5 in shoots of Zea mays L

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.1.127· OSTI ID:5946296

Fujioka, Shozo; Spray, C R; Phinney, B O ^[1]; Gaskin, P; MacMillan, J ^[2]; Yamane, Hisakazu ^[3]; Takahashi, Nobutaka ^[4]

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1. Univ. of California, Los Angeles (USA)
2. Univ. of Bristol (England)
3. Univ. of California, Los Angeles (USA) Univ. of Tokyo (Japan)
4. Univ. of Tokyo (Japan)

(17-{sup 13}C, {sup 3}H)-Labeled gibberellin A{sub 20} (GA{sub 20}), GA{sub 5}, and GA{sub 1} were fed to homozygous normal (+/+), heterozygous dominant dwarf (D8/+), and homozygous dominant dwarf (D8/D8) seedlings of Zea mays L. (maize). {sup 13}C-Labeled GA{sub 29}, GA{sub 8}, GA{sub 5}, GA{sub 1}, and 3-epi-GA{sub 1}, as well as unmetabolized ({sup 13}C)GA{sub 20}, were identified by gas chromatography-selected ion monitoring (GC-SIM) from feeds of (17-{sup 13}C, {sup 3}H)GA{sub 20} to all three genotypes. {sup 13}C-Labeled GA{sub 8} and 3-epi-G{sub 1}, as well as unmetabolized ({sup 13}C)GA{sub 1}, were identified by GC-SIM from feeds of (17-{sup 13}C, {sup 3}H)GA{sub 1} to all three genotypes. From feeds of (17-{sup 13}C, {sup 3}H)GA{sub 5}, {sup 13}C-labeled GA{sub 3} and the GA{sub 3}-isolactone, as well as unmetabolized ({sup 13}C)GA{sub 5}, were identified by GC-SIM from +/+ and D8/D8, and by full scan GC-MS from D8/+. No evidence was found for the metabolism of (17-{sup 13}C, {sup 3}H)GA{sub 5} to ({sup 13}C)GA{sub 1}, either by full scan GC-mass spectrometry or by GC-SIM. The results demonstrate the presence in maize seedlings of three separate branches from GA{sub 20}, as follows: (a) GA{sub 20} {yields} GA{sub 1} {yields} GA{sub 8}; (b) GA{sub 20} {yields} GA{sub 5} {yields} GA{sub 3}; and (c) GA{sub 20} {yields} GA{sub 29}. The in vivo biogenesis of GA{sub 3} from GA{sub 5}, as well as the origin of GA{sub 5} from GA{sub 20}, are conclusively established for the first time in a higher plant (maize shoots).

• OSTI ID: 5946296
• Journal Information: Plant Physiology; (USA), Vol. 94:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
BIOSYNTHESIS
CARBON 13
CARBON COMPOUNDS
IN VIVO
MAIZE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CARBON ISOTOPES
CARBOXYLIC ACIDS
CEREALS
ESTERS
EVEN-ODD NUCLEI
GRASS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LACTONES
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
STABLE ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques