Biosynthesis of sulfoquinovosyldiacylglycerol in higher plants
Intact spinach chloroplasts incorporated /sup 35/SO/sub 4//sup 2 -/ into sulfoquinovosyldiacylglycerol in the dark at rates equivalent to those previously reported for illuminated chloroplasts provided that either ATP itself or an ATP-generating system was added. No additional reductant was necessary for SQDG synthesis by chloroplasts. The optimal concentration of ATP was between 2 and 3 millimolar. Rates of synthesis up to 2.6 nanomoles per milligram chlorophyll per hour were observed. UTP, GTP, and CTP could not substitute for ATP. Incubation of UTP with ATP (1:1) stimulated synthesis of sulfoquinovosyldiacylglycerol. No additional stimulation of the reaction was observed upon addition of other nucleoside triphosphates with ATP. For the generation of ATP in the chloroplast, addition of dihydroxyacetone phosphate alone did not promote synthesis of sulfoquinovosyldiacylglycerol, but in combination with inorganic phosphate and oxaloacetate, rates of synthesis up to 3.2 nanomoles per milligram chlorophyll per hour were observed. Dark synthesis was optimal in the presence of 2 millimolar dihydroxyacetone phosphate, 2 millimolar oxaloacetate, and 1 millimolar KH/sub 2/PO/sub 4/.
- Research Organization:
- Plant Cell Research Institute, Dublin, CA
- OSTI ID:
- 5905076
- Journal Information:
- Plant Physiol.; (United States), Vol. 84:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPIDS
BIOSYNTHESIS
ORGANIC SULFUR COMPOUNDS
VISIBLE RADIATION
BIOLOGICAL EFFECTS
ANIONS
ATP
BIOCHEMICAL REACTION KINETICS
CHLOROPLASTS
LABELLED COMPOUNDS
NUCLEOSIDES
PHOTOSYNTHETIC MEMBRANES
SPINACH
STIMULATION
SULFATES
SULFUR 35
TRACER TECHNIQUES
UTP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
EVEN-ODD NUCLEI
FOOD
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANES
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RIBOSIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VEGETABLES
550201* - Biochemistry- Tracer Techniques