Retrovirus transduction: Segregation of the viral transforming function and the Herpes Simplex virus tk gene in infectious friend spleen focus-forming virus thymidine kinase vectors
A series of deletions and insertions utilizing the herpesvirus thymidine kinase gene (tk) were constructed in the murine retrovirus Friend spleen focus-forming virus (SFFV). In all cases, the coding region for the SFFV-specific glycoprotein (gp55), which is implicated in erythroleukemic transformation, was left intact. These SFFV-TK and SFFV deletion vectors were analyzed for expression of tk and gp55 after DNA-mediated gene transfer. In addition, virus rescued by cotranfection of these vectors with Moloney murine leukemia virus was analyzed for infectious TK-transducing virus, gp55 expression, and erythroleukemia-inducing ability. The experiments demonstrated that deletions or insertions within the intron for the gp55 env gene can interfere with expression of gp55 after both DNA-mediated gene transfer and virus infection. In contrast, the gene transfer efficiency of the tk gene was unaffected in the SFFV-TK vectors, and high-titer infectious TK virus could be recovered. Revertant viruses capable of inducing erythroleukemia and expressing gp55 were generated after cotranfection of the SFFV-TK vectors with murine leukemia virus. The revertant viruses lost both tk sequences and the ability to transduce TK/sup -/ fibroblasts to a TK/sup +/ phenotype. These experiments demonstrate that segregation of the TK and erythroleukemia functions can occur in retrovirus vectors which initially carry both markers.
- Research Organization:
- Ontario Cancer Institute and Dept. of Medical Biophysics, Univ. of Toronto, Toronto, Ontario M4X 1K9
- OSTI ID:
- 5891557
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 3:12
- Country of Publication:
- United States
- Language:
- English
Similar Records
Oncogenicity of human N-ras oncogene and proto-oncogene introduced into retroviral vectors
Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene
Related Subjects
HERPES SIMPLEX
MUTAGENESIS
LEUKEMIA VIRUSES
REVERTANTS
ONCOGENES
DNA SEQUENCING
PHOSPHOTRANSFERASES
GENES
GENETIC ENGINEERING
CHROMOSOME LOSSES
ERYTHROCYTES
FIBROBLASTS
GLUCOPROTEINS
ONCOGENIC TRANSFORMATIONS
PHENOTYPE
RECOMBINANT DNA
RNA PROCESSING
SPLEEN
STRUCTURAL MODELS
THYMIDINE
VIRUSES
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ENZYMES
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LOSSES
MATERIALS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SKIN DISEASES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRAL DISEASES
550400* - Genetics