Determination of cyanide using a microbial sensor

Journal Article · Thu Aug 01 00:00:00 EDT 1996 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02788064· OSTI ID:588725

Nakanishi, Keijiro; Ikebukuro, Kazunori; Karube, Isao ^[1]

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1. Univ. of Tokyo (Japan)

A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrode. When cyanide is added to the sample solution, the electron transport chain reaction of the respiration system in the mitochondria is inhibited, resulting in a decrease in respiration. The inhibition is caused by cyanide binding with respiration enzymes such as the cytochrome oxidase complex in the mitochondrial inner membrane. Therefore, the cyanide concentration can be measured from the change in the respiration rate. When the sensor was applied to a batch system at pH 8.0 and 30{degrees}C, the cyanide calibration curve showed linearity in the concentration range between 0.3 pM and 150 {mu}m CN{sup -}. 13 refs., 8 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 588725
• Journal Information: Applied Biochemistry and Biotechnology, Vol. 60, Issue 2; Other Information: PBD: Aug 1996
• Country of Publication: United States
• Language: English

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CALIBRATION
CYTOCHROME OXIDASE
ENZYMES
GLUCOSE
MICROORGANISMS
RANGE
RESPIRATION
PH VALUE
TEMPERATURE DEPENDENCE
SACCHAROMYCES CEREVISIAE
CYANIDES
DETECTION
MONITORING
ELECTRODES
CONCENTRATION RATIO
CORRELATIONS
WASTE PROCESSING
TOXIC MATERIALS
SEPARATION PROCESSES