Functional compartmentation of the nucleotide pool
Various lines of evidence show that the total cellular ribonucleoside triphosphate pool serves as the percursor pool for RNA synthesis. Other reports strongly support the thesis that the ribonucleotide pool is compartmentalized. In particular, the suggestion has been put forward that, in some cell lines, nucleosides such as uridine are rapidly channeled into the putative functional pool. The present experiments were designed to obtain a more direct answer to the question of nucleotide pool compartmentation. From these data, one can assess the feasibility of using specific activities of the total pool nucleoside triphosphates for calculating rates of RNA synthesis. Two cell lines were used in this investigation. A rat transformed tracheal cell line, cloned from a keratinizing squamous cell carcinoma, was grown as a stratified epithelium, and Novikoff hepatoma cell line, grown in suspension culture. Tritiated nucleoside and /sup 32/PO/sub 4/ were added to cells in exponential growth. Under the conditions used, between 85 to 100% of the RNA is hydrolyzed to 5'-mononucleotides. Furthermore, the enzyme was shown to be almost free of 5'-nucleotidase and totally devoid of deaminase and phosphoryl-transferring activities. (ERB)
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5880754
- Report Number(s):
- CONF-811057-1; ON: DE82001497
- Resource Relation:
- Conference: EPA/HERL international symposium on health effects of tumor promotion, Cincinnati, OH, USA, 13 Oct 1981
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NUCLEOTIDES
COMPARTMENTS
RNA
BIOSYNTHESIS
CELL CULTURES
CLONE CELLS
HEPATOMAS
LABELLED COMPOUNDS
NUCLEOSIDES
PHOSPHORUS 32
PRECURSOR
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RIBOSIDES
RODENTS
SYNTHESIS
VERTEBRATES
550200* - Biochemistry
550300 - Cytology