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Title: Modifications of und rbcS in und Synechococcus sp. PCC7942 resulted in high CO sub 2 requiring mutants

High CO{sub 2} requiring mutants (HCR), capable of growing in the presence of kanamycin, were obtained following transformation with a construct containing {und nptII} in the coding region of rbcS (encoding for the small subunit (SS) of Rubisco). The apparent photosynthetic affinity for inorganic carbon was very low in mutant EK6 whereas in mutant JR12 it was only slightly lower than in high-CO{sub 2}-grown wild type. Southern analysis indicated double crossing over in the case of EK6. In mutant JR12 Southern analysis revealed some rearrangement and a deletion of a region downstream fro {und rbcS}. Western analysis demonstrated normal SS in JR12 but a larger (by about 3 kD) SS in EK6. Sequence analysis indicated that the latter was the result of fusion between {und rbcS} and the flanking region of {und nptII}.
Authors:
; ; ; ;  [1]
  1. (Hebrew Univ. of Jerusalem (Israel))
Publication Date:
OSTI Identifier:
5841377
Report Number(s):
CONF-9007196--
Journal ID: ISSN 0079-2241; CODEN: PPYSA
Resource Type:
Conference
Resource Relation:
Journal Name: Plant Physiology, Supplement; (USA); Journal Volume: 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALGAE; MUTANTS; CARBON DIOXIDE; METABOLISM; PHOTOSYNTHESIS; RIBULOSE DIPHOSPHATE CARBOXYLASE; CARBON COMPOUNDS; CARBON OXIDES; CARBON-CARBON LYASES; CARBOXY-LYASES; CHALCOGENIDES; CHEMICAL REACTIONS; ENZYMES; LYASES; OXIDES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; SYNTHESIS 550500* -- Metabolism