Synthesis of calmodulin-binding proteins during heat shock in tobacco cells

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5841297

Lu, Yingtang; Harrington, M ^[1]

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1. Univ. of Hawaii, Honolulu (USA)

Heat shock treatment induces the synthesis of heat shock proteins (HSPs), but little is known about the functions of these proteins in the heat shock response. Here we report isolation and analysis of heat-shock induced or enhanced calmodulin-binding proteins (CaMBPs) from cultured tobacco cells (Nicotiana tabacum cv. Wisconsin-38) using CaM-sepharose affinity chromatography. Analyses of {sup 35}S-methionine-labeled proteins by SDS-PAGE indicate that at least 12 HSP bands with apparent molecular weights ranging from 105 to 17 kD exhibit Ca{sup 2+}-dependent binding to CaM sepharose even in the presence of 0.3M NaCl. Thee proteins do not bind to sepharose 4B suggesting a specific interaction with CaM. Gel overlay analysis of HSPs binding to CaM-sepharose indicates that not all of these peptides bind to {sup 125}I-CaM in this assay. This may be due to the structural modification of {sup 125}I-CaM, the resolution of the assay, or the small amount of the CaMBPs synthesized during heat shock. An alternative approach is being employed using {sup 35}S-labeled CaM made from a synthetic CaM gene (VUC-1) to confirm the CaMBP/HSP by overlay analysis and to screen a heat shock cDNA expression library.

• OSTI ID: 5841297
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALMODULIN
METABOLISM
NICOTIANA
BIOLOGICAL STRESS
CELL CULTURES
SULFUR 35
SULFUR COMPOUNDS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
550501* - Metabolism- Tracer Techniques