Dynamics of interaction between complement-fixing antibody/dsDNA immune complexes and erythrocytes. In vitro studies and potential general applications to clinical immune complex testing
Soluble antibody//sub 3/H-double-stranded PM2 DNA (dsDNA) immune complexes were briefly opsonized with complement and then allowed to bind to human erythrocytes (via complement receptors). The cells were washed and subsequently a volume of autologous blood in a variety of media was added, and the release of the bound immune complexes from the erythrocytes was studied as a function of temperature and time. After 1-2 h, the majority of the bound immune complexes were not released into the serum during blood clotting at either 37 degrees C or room temperature, but there was a considerably greater release of the immune complexes into the plasma of blood that was anticoagulated with EDTA. Similar results were obtained using various conditions of opsonization and also using complexes that contained lower molecular weight dsDNA. Thus, the kinetics of release of these antibody/dsDNA immune complexes differed substantially from the kinetics of release of antibody/bovine serum albumin complexes that was reported by others. Studies using the solution phase C1q immune complex binding assay confirmed that in approximately half of the SLE samples that were positive for immune complexes, there was a significantly higher level of detectable immune complexes in plasma vs. serum. Freshly drawn erythrocytes from some SLE patients exhibiting this plasma/serum discrepancy had IgG antigen on their surface that was released by incubation in EDTA plasma. Thus, the higher levels of immune complexes observed in EDTA plasma vs. serum using the C1q assay may often reflect the existence of immune complexes circulating in vivo bound to erythrocytes.
- Research Organization:
- Univ. of Virginia, Charlottesville, VA (United States)
- OSTI ID:
- 5829077
- Journal Information:
- J. Clin. Invest.; (United States), Vol. 75:1
- Country of Publication:
- United States
- Language:
- English
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COMPLEXES
BIOCHEMICAL REACTION KINETICS
LUPUS
IMMUNOLOGY
ANTIGEN-ANTIBODY REACTIONS
BLOOD PLASMA
BLOOD SERUM
DNA
EDTA
ERYTHROCYTES
PATIENTS
RECEPTORS
RESPONSE MODIFYING FACTORS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
DISEASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SKIN DISEASES
TUBERCULOSIS
550201* - Biochemistry- Tracer Techniques