sup 31 P saturation transfer and phosphocreatine imaging in the monkey brain

Journal Article · Tue Oct 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.19.8372· OSTI ID:5822076

Mora, B; Narasimhan, P T; Ross, B D ^[1]; Allman, J ^[2]; Barker, P B ^[3]

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1. Huntington Medical Research Inst., Pasadena, CA (United States) California Inst. of Tech., Pasadena (United States)
2. California Inst. of Tech., Pasadena (United States)
3. Huntington Medical Research Inst., Pasadena, CA (United States)

{sup 31}P magnetic resonance imaging with chemical-shift discrimination by selective excitation has been employed to determine the phosphocreatine (PCr) distribution in the brains of three juvenile macaque monkeys. PCr images were also obtained while saturating the resonance of the {gamma}-phosphate of ATP, which allowed the investigation of the chemical exchange between PCr and the {gamma}-phosphate of ATP catalyzed by creatine kinase. Superposition of the PCr images over the proton image of the same monkey brain revealed topological variations in the distribution of PCr and creatine kinase activity. PCr images were also obtained with and without visual stimulation. In two out of four experiments, an apparently localized decrease in PCr concentration was noted in visual cortex upon visual stimulation. This result is interpreted in terms of a possible role for the local ADP concentration in stimulating the accompanying metabolic response.

• OSTI ID: 5822076
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:19; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOCREATINE
NUCLEAR MAGNETIC RESONANCE
PHOSPHOTRANSFERASES
TISSUE DISTRIBUTION
BRAIN
FEMALES
MONKEYS
PHOSPHORUS 31
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
ENZYMES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
RESONANCE
STABLE ISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques