skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands

Abstract

A unique type of radioimmunoassay is described that does not require centrifugation or separation. Microbeads containing a fluorophor are covalently linked to antibody. When an /sup 125/I-labeled antigen is added it binds to the beads and, by its proximity, the emitted short-range electrons of the /sup 125/I excite the fluorophor in the beads. The light emitted can be measured in a standard scintillation counter. Addition of unlabeled antigen from tissue extracts displaces the labeled ligand and diminishes the fluorescent signal. Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described. Applications of the principle to study the kinetics of interaction between receptors and ligands are discussed.

Authors:
; ; ;
Publication Date:
Research Org.:
Roche Institute of Molecular Biology, Nutley, NJ
OSTI Identifier:
5808400
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 24
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ENKEPHALINS; RADIOIMMUNOASSAY; MORPHINE; THYROXINE; IODINE 125; LABELLED COMPOUNDS; SCINTILLATION COUNTING; ALKALOIDS; AMINO ACIDS; ANALGESICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM DEPRESSANTS; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENDORPHINS; HORMONES; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NARCOTICS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; OPIUM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Udenfriend, S, Gerber, L D, Brink, L, and Spector, S. Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands. United States: N. p., 1985. Web. doi:10.1073/pnas.82.24.8672.
Udenfriend, S, Gerber, L D, Brink, L, & Spector, S. Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands. United States. https://doi.org/10.1073/pnas.82.24.8672
Udenfriend, S, Gerber, L D, Brink, L, and Spector, S. 1985. "Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands". United States. https://doi.org/10.1073/pnas.82.24.8672.
@article{osti_5808400,
title = {Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands},
author = {Udenfriend, S and Gerber, L D and Brink, L and Spector, S},
abstractNote = {A unique type of radioimmunoassay is described that does not require centrifugation or separation. Microbeads containing a fluorophor are covalently linked to antibody. When an /sup 125/I-labeled antigen is added it binds to the beads and, by its proximity, the emitted short-range electrons of the /sup 125/I excite the fluorophor in the beads. The light emitted can be measured in a standard scintillation counter. Addition of unlabeled antigen from tissue extracts displaces the labeled ligand and diminishes the fluorescent signal. Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described. Applications of the principle to study the kinetics of interaction between receptors and ligands are discussed.},
doi = {10.1073/pnas.82.24.8672},
url = {https://www.osti.gov/biblio/5808400}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 24,
place = {United States},
year = {Sun Dec 01 00:00:00 EST 1985},
month = {Sun Dec 01 00:00:00 EST 1985}
}