Vitamin E alters alveolar type II cell phospholipid synthesis in oxygen and air

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Experimental Lung Research; (USA)

DOI:https://doi.org/10.3109/01902149009087883· OSTI ID:5803368

Kennedy, K A; Snyder, J M; Stenzel, W; Saito, K; Warshaw, J B ^[1]

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1. Univ. of Texas Southwestern Medical School, Dallas (USA)

Newborn rats were injected with vitamin E or placebo daily until 6 days after birth. The effect of vitamin E pretreatment on in vitro surfactant phospholipid synthesis was examined in isolated type II cells exposed to oxygen or air form 24 h in vitro. Type II cells were also isolated from untreated 6-day-old rats and cultured for 24 h in oxygen or air with control medium or vitamin E supplemented medium. These cells were used to examine the effect of vitamin E exposure in vitro on type II cell phospholipid synthesis and ultrastructure. Phosphatidylcholine (PC) synthesis was reduced in cells cultured in oxygen as compared with air. This decrease was not prevented by in vivo pretreatment or in vitro supplementation with vitamin E. Vitamin E pretreatment increased the ratio of disaturated PC to total PC and increased phosphatidylglycerol synthesis. The volume density of lamellar bodies in type II cells was increased in cells maintained in oxygen. Vitamin E did not affect the volume density of lamellar bodies. We conclude that in vitro hyperoxia inhibits alveolar type II cell phosphatidylcholine synthesis without decreasing lamellar body volume density and that supplemental vitamin E does not prevent hyperoxia-induced decrease in phosphatidylcholine synthesis.

• OSTI ID: 5803368
• Journal Information: Experimental Lung Research; (USA), Vol. 16:6; ISSN 0190-2148
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOLIPIDS
BIOSYNTHESIS
VITAMIN E
BIOLOGICAL EFFECTS
CELL CULTURES
CHOLINE
OXYGEN
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
DRUGS
ELEMENTS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
NONMETALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
QUATERNARY COMPOUNDS
RODENTS
SYNTHESIS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques