An antioxidant agent prevents NO sub 2 -induced inhibition of mast cell mediator release: Evidence that the mechanism involves free radicals

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Environmental Research; (USA)

DOI:https://doi.org/10.1016/S0013-9351(05)80117-3· OSTI ID:5799487

Fujimaki, Hidekazu; Shiraishi, Fujio; Wakamori, Kazuo ^[1]

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1. National Institute for Environmental Studies, Ibaraki (Japan) Jikei Univ. School of Medicine, Tokyo (Japan)

Previously we established that in vitro NO{sub 2} exposure induced inhibition of histamine release from rat peritoneal mast cells (PMC) stimulated with secretagogues such as compound 48/80 or substance P. To further explore the effects of NO{sub 2} exposure on mast cells, we investigated whether the addition of an antioxidant agent, 2-mercaptoethanol (2-ME), can prevent NO{sub 2}-induced inhibition of mediator release from PMC. Histamine release from 5 ppm NO{sub 2}-exposed PMC stimulated with 20 {mu}M substance P was also significantly inhibited compared with that from the controls. {beta}-Hexosaminidase release from 5 ppm NO{sub 2}-exposed PMC stimulated with 20 {mu}M substance P was also significantly inhibited. However, the inhibition of both histamine and {beta}-hexosaminidase release from exposed PMC was diminished by the addition of 5 mM 2-ME during NO{sub 2} exposure. Although IgE-mediated histamine release from NO{sub 2} exposed PMC was markedly inhibited, the addition of 5 mM 2-ME during NO{sub 2} exposure induced no inhibition of histamine release. These results suggest a possible relationship between NO{sub 2}-induced inhibition of mast cell mediator release and production of free radicals by the action of NO{sub 2}.

• OSTI ID: 5799487
• Journal Information: Environmental Research; (USA), Vol. 53:2; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIOXIDANTS
BIOLOGICAL EFFECTS
NITROGEN DIOXIDE
TOXICITY
ANIMAL CELLS
HISTAMINE
MAST CELLS
RADICALS
RATS
AMINES
ANIMALS
AZOLES
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MAMMALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology