Isolation and characterization of a catabolite repression-insensitive mutant of a methanol yeast, Candida boidinii A5, producing alcohol oxidase in glucose-containing medium

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5777309

Sakai, Y; Sawai, T; Tani, Y

Mutants exhibiting alcohol oxidase activity when grown on glucose in the presence of methanol were found among 2-deoxyglucose-resistant mutants derived from a methanol yeast, Candida boidinii A5. One of these mutants, strain ADU-15, showed the highest alcohol oxidase activity in glucose-containing medium. The growth characteristics and also the induction and degradation of alcohol oxidase were compared with the parent strain and mutant strain ADU-15. In the parent strain, initiation of alcohol oxidase synthesis was delayed by the addition of 0.5% glucose to the methanol medium, whereas it was not delayed in mutant strain ADU-15. This showed that alcohol oxidase underwent repression by glucose. On the other hand, degradation of alcohol oxidase after transfer of the cells from methanol to glucose medium (catabolite inactivation) was observed to proceed at similar rates in parent and mutant strains. The results of immunochemical titration experiments suggests that catabolite inactivation of alcohol oxidase is coupled with a quantitative change in the enzyme. Mutant strain ADU-15 was proved to be a catabolite repression-insensitive mutant and to produce alcohol oxidase in the presence of glucose. However, it was not an overproducer of alcohol oxidase and, in both the parent and mutant strains, alcohol oxidase was completely repressed by ethanol.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 5777309
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 53:8
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CANDIDA
GROWTH
GLUCOSE
METABOLISM
METHANOL
OXIDOREDUCTASES
ENZYME ACTIVITY
MUTANTS
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
ENZYMES
FUNGI
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
YEASTS
560300* - Chemicals Metabolism & Toxicology
550700 - Microbiology