Primary gene products as plant defenses. Final report, November 1, 1983-October 31, 1984

Technical Report · Tue Apr 01 00:00:00 EST 1986

OSTI ID:5767416

Murdock, L L

Using the bruchid beetle, Callosobruchus maculatus, as a model insect we sought to understand the effects of proteinaceous inhibitors of insect digestive proteinases on growth and development of a representative pest species. We found that C. maculatus has a major migut proteinase of the thiol type which is powerfully inhibited by E-64. We devised a technique for preparing and incorporating proteinases inhibitors into artificial cowpeas: E-64, at concentrations as low as 0.01% (w/w), slowed development, increased mortality, and reduced fecundity. Clearly, thiol proteinase inhibitors in the diet of C. maculatus have a severe impact on this insect and could prevent economic infestations from developing were such inhibitors present in their natural food, i.e., cowpeas.

• Research Organization: Purdue Univ., Lafayette, IN (USA). Dept. of Entomology
• DOE Contract Number: AC02-80ER10715
• OSTI ID: 5767416
• Report Number(s): DOE/ER/10715-1; ON: DE86010327
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ENZYME INHIBITORS
FRACTIONATION
SH-PROTEINASES
BEETLES
GEL PERMEATION CHROMATOGRAPHY
GENETIC ENGINEERING
GLYCINE HISPIDA
ANIMALS
ARTHROPODS
BACTERIA
CHROMATOGRAPHY
COLEOPTERA
ENZYMES
HYDROLASES
INSECTS
INVERTEBRATES
LEGUMINOSAE
MICROORGANISMS
PEPTIDE HYDROLASES
PLANTS
RHIZOBIUM
SEPARATION PROCESSES
550200* - Biochemistry
553000 - Agriculture & Food Technology