Lea blood group antigen on human platelets
One- and two-stage radioligand assays were used to determine if human platelets possess the Lea antigen. Goat IgG anti-Lea antibody was purified by multiple adsorptions with Le(a-b-) human red blood cells, followed by affinity chromatography with synthetic Lea substance and labeling with /sup 125/I. Human IgG anti-Lea antibody was used either in a two stage radioassay with /sup 125/I-labeled mouse monoclonal IgG anti-human IgG as the second antibody or, alternatively, purified by Staph protein A chromatography, labeled with /sup 125/I, and used in a one-stage radioassay. Platelets from donors of appropriate red blood cell phenotypes were incubated with the antisera, centrifuged through phthalate esters, and assayed in a gamma scintillation counter. Dose response and saturation curve analysis demonstrate the presence of Lewis a antigen on platelets from Lea+ donors. Furthermore, platelets from an Le(a-b-) donor incubated in Le (a+b-) plasma adsorb Lea antigen in a similar manner to red blood cells. The clinical significance of these antigens in platelet transfusion remains undefined.
- Research Organization:
- Univ. Medical Center, Durham, NC
- OSTI ID:
- 5750011
- Journal Information:
- Am. J. Clin. Pathol.; (United States), Vol. 83:1
- Country of Publication:
- United States
- Language:
- English
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BLOOD PLATELETS
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ERYTHROCYTES
GOATS
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LABELLED COMPOUNDS
LIGANDS
MONOCLONAL ANTIBODIES
SCINTILLATION COUNTERS
TRACER TECHNIQUES
TRANSFUSIONS
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BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
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BLOOD CELLS
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DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
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MATERIALS
MEASURING INSTRUMENTS
NUCLEI
ODD-EVEN NUCLEI
RADIATION DETECTORS
RADIOASSAY
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551001* - Physiological Systems- Tracer Techniques