Interindividual variation with respect to DNA repair in human cells

Conference · Sun Jan 01 00:00:00 EST 1989

OSTI ID:5719421

Leonard, R C; Leonard, J C; Bender, M A; Wieland, J; Setlow, R B

Ecogenetics is the study of genetically determined differences among individuals in their susceptibility to the actions of physical, chemical, and biological agents in the environment. An individual's most basic level of response to these environmental agents may be the ability to repair physical and chemical damage to DNA. We have been engaged in a survey of DNA-repair measurements in a healthy working population in order to determine the extent of the population variability in these endpoints and to assess the value of these screening protocols in identifying individuals who are at the extremes of the distribution. In addition, we are measuring intraindividual variation over time, as well as the correlations between measurements of different repair systems. The endpoints that we have chosen to use are cytogenetic responses (SCE's and micronucleus formation) and DNA excision repair (unscheduled DNA synthesis and removal of O{sup 6} guanine methylation) in human peripheral lymphocytes exposed to 254 nm ultraviolet light, x-rays, the bifunctional alkylating agent mitomycin C, or the monofunctional alkylating agent N-methyl-N-nitro-nitrosoguanidine (MNNG). These four test mutagens produce spectra of DNA lesions eliciting different types of DNA repair. 3 refs., 1 tab.

• Research Organization: Brookhaven National Lab., Upton, NY (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5719421
• Report Number(s): BNL-42309; CONF-891072-1; ON: DE89008178
• Resource Relation: Conference: 27. Hanford symposium on health and the environment: multilevel research--molecules to man, Richland, WA (USA), 18-20 Oct 1989
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL VARIABILITY
EVALUATION
POPULATION DYNAMICS
TESTING
AUTORADIOGRAPHY
DNA REPAIR
INVENTIONS
LYMPHOCYTES
MAN
MUTAGENS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAMMALS
MATERIALS
PRIMATES
RECOVERY
REPAIR
SOMATIC CELLS
VERTEBRATES
550200* - Biochemistry