Growth stimulation of 3T3 fibroblasts by Cystatin

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Experimental Cell Research; (United States)

OSTI ID:5715026

Sun, Quan ^[1]

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1. Michigan State Univ., East Lansing (United States) Beijing Medical Univ. (China)

Treatment of cultures of mouse 3T3 fibroblasts with Cystatin C, a thiol-proteinase inhibitor isolated from chicken egg white, resulted in an enhanced rate of cell proliferation. This stimulation was demonstrated using two independent assay systems: (a) assessment of total cell number and (b) measurement of ({sup 3}H)thymidine incorporated into acid-precipitable DNA. In both assays, the dose-response curves of Cystatin stimulation showed a rising function that plateaued at a concentration of {approximately}120 {mu}g/ml. The addition of Cystatin to cultures of Kirsten murine sarcoma virus-transformed 3T3 cells also enhanced DNA synthesis in these target cells. Control experiments showed that the presence of Cystatin did not alter the level of binding of radioactively labeled epidermal growth factor and platelet derived growth factor to 3T3 cells. These results argue against the possibility that the observed growth stimulation by Cystatin was due to growth factor contamination of the Cystatin preparation.

• OSTI ID: 5715026
• Journal Information: Experimental Cell Research; (United States), Vol. 180:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME INHIBITORS
BIOLOGICAL EFFECTS
FIBROBLASTS
CELL PROLIFERATION
GROWTH FACTORS
RADIORECEPTOR ASSAY
DOSE-RESPONSE RELATIONSHIPS
IODINE 125
PEPTIDE HYDROLASES
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MITOGENS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SOMATIC CELLS
TRACER TECHNIQUES
550301* - Cytology- Tracer Techniques