Propionate induces cell swelling and K+ accumulation in shark rectal gland
- Mount Desert Island Biological Laboratory, Salsbury Cove, ME (USA)
Small organic anions have been reported to induce cell solute accumulation and swelling. To investigate the mechanism of swelling, we utilized preparations of rectal gland cells from Squalus acanthias incubated in medium containing propionate. Propionate causes cells to swell by diffusing across membranes in its nonionic form, acidifying cell contents, and activating the Na+-H+ antiporter. The Na+-H+ exchange process tends to correct intracellular pH (pHi), and thus it maintains a favorable gradient for propionic acid diffusion and allows propionate to accumulate. Activation of the Na+-H+ antiport also facilitates Na+ entry into the cell and Nai accumulation. At the same time Na+-K+-ATPase activity, unaffected by propionate, replaces Nai with Ki, whereas the K+ leak rate, decreased by propionate, allows Ki to accumulate. As judged by {sup 86}Rb+ efflux, the reduction in K+ leak was not due to propionate-induced cell acidification or reduction in Cli concentration. Despite inducing cell swelling, propionate did not disrupt cell structural elements and F actin distribution along cell membranes.
- OSTI ID:
- 5673296
- Journal Information:
- American Journal of Physiology; (USA), Vol. 257; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
PATHOLOGICAL CHANGES
POTASSIUM COMPOUNDS
BIOLOGICAL ACCUMULATION
PROPIONIC ACID
BIOCHEMICAL REACTION KINETICS
ACTIN
ATP
CELL MEMBRANES
EPITHELIUM
FISHES
GLANDS
IN VITRO
OUABAIN
PROTEINS
RECTUM
RUBIDIUM
RUBIDIUM 86
SKIN
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
GASTROINTESTINAL TRACT
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LARGE INTESTINE
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
RUBIDIUM ISOTOPES
STROPHANTHINS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques