Novel methods for determination of the trichothecene mycotoxin deoxynivalenol

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5623312

Sylvia, Jr, V L

A rapid method for analysis of deoxynivalenol (DON) was developed using high pressure liquid chromatography with reductive electrochemical detection (LCECD). Deoxynivalenol produced by Fusarium roseum growing on solid cornmeal and rice substrates and from naturally contaminated wheat was extracted and quantitated. Two deoxynivalenol producing isolates of Fusarium roseum (ATCC number28114, NCPRL-A) were incubated in stationary liquid culture in the presence of (1-/sup 14/C) sodium acetate to biosynthetically produce a radiolabeled toxin suitable for use in tissue distribution studies. Specific activities of 139 uCi/mmole (NCPRL-A on corn media), 67.5 uCi/mmole (NCPRL-A on rice media) and 21.5 uCi/mmol (ATCC number28114 on rice media) were obtained in this study and represent the first reported incorporation of /sup 14/C into deoxynivalenol. A minicolumn method for DON determination was also investigated.

• Research Organization: Texas A and M Univ., College Station (USA)
• OSTI ID: 5623312
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
TISSUE DISTRIBUTION
TOXINS
ELECTROCHEMISTRY
LIQUID COLUMN CHROMATOGRAPHY
ACETATES
FUSARIUM
MAIZE
RICE
WHEAT
ANTIGENS
CARBOXYLIC ACID SALTS
CEREALS
CHEMISTRY
CHROMATOGRAPHY
DISTRIBUTION
FUNGI
GRASS
LABELLED COMPOUNDS
MATERIALS
PARASITES
PLANTS
SEPARATION PROCESSES
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques