Assay of cysteine dioxygenase activity
- Cornell Univ., Ithaca, NY (United States)
It has been proposed that rat liver contains two cysteine dioxygenase enzymes which convert cysteine to cysteinesulfinic acid, one which is stimulated by NAD{sup +} and has a pH optimum of 6.8 and one which is not stimulated by NAD{sup +} and has a pH optimum of 9.0. This led the authors to reinvestigate assay conditions for measuring cysteine dioxygenase activity in rat liver homogenate. An HPLC method, using an anion exchange column (Dionex Amino-Pac{trademark} PA1 (4x250 mm)) was used to separate the ({sup 35}S)cysteinesulfinic acid produced from ({sup 35}S)cysteine in the incubation mixture. They demonstrated that inclusion of hydroxylamine prevented further metabolism of cysteinesulfinic acid. which occurred rapidly in the absence of hydroxylamine.
- OSTI ID:
- 5618638
- Report Number(s):
- CONF-9004153-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 74. annual meeting of the Federation of American Societies for Experimental Biology, Washington, DC (United States), 1-5 Apr 1990; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIVER
ENZYME ACTIVITY
OXYGENASES
BIOCHEMICAL REACTION KINETICS
CYSTEINE
HOMOGENATES
RATS
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYMES
EVEN-ODD NUCLEI
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR ISOTOPES
THIOLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques