Ultraviolet light-resistant primary transfectants of xeroderma pigmentosum cells are also DNA repair-proficient
Journal Article
·
· Biochemical and Biophysical Research Communications; (USA)
- Tel Aviv Univ., Ramat Aviv (Israel)
In a previous work, an immortal xeroderma pigmentosum cell line belonging to complementation group C was complemented to a UV-resistant phenotype by transfection with a human cDNA clone library. We now report that the primary transformants selected for UV-resistance also acquired normal levels of DNA repair. This was assessed both by measurement of UV-induced ({sup 3}H)thymidine incorporation and by equilibrium sedimentation analysis of repair-DNA synthesis. Therefore, the transduced DNA element which confers normal UV-resistance also corrects the excision repair defect of the xeroderma pigmentosum group C cell line.
- OSTI ID:
- 5601875
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Vol. 162:3; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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+3 more
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
XP CELLS
DNA REPAIR
RADIOSENSITIVITY
CLONING
IN VITRO
MAN
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SKIN DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
XP CELLS
DNA REPAIR
RADIOSENSITIVITY
CLONING
IN VITRO
MAN
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SKIN DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques