Comparison of procedures for preparation of Pinus strobus needle macromolecules

Conference · Wed May 01 00:00:00 EDT 1991 · Plant Physiology, Supplement; (United States)

OSTI ID:5561112

Williams, A L; Dashek, W V ^[1]; Vose, J M; Swank, W T ^[2]

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1. Clark Atlanta Univ., GA (United States)
2. Dept. of Agriculture, Otto, NC (United States)

Eastern white pine foliage is sensitive to adverse atmospheric O{sub 3} and acid rain. The mechanisms by which they promote needle necrosis have not been fully elucidated. Because the literature yielded little regarding needle protein and nucleic acid contents, streptomycin sulfate (SS) and trichloroacetic acid (TCA) efficiencies for macromolecule precipitation from 1 and 2 yr-old needles of 30 yr-old trees were compared. Weighed needles were homogenized into buffer and homogenates filtered. The filtrate was mixed with chloroform-methanol-H{sub 2}O (13:4:3, v/v) and centrifuged 30 min 12,000 xg. After the pellet was subjected to SS or TCA, Lowry-positive substances were recovered in SS and TCA-soluble and insoluble fractions of 1st and 2nd yr needles. Similarly, orcinol and diphenylamine-positive substances occurred in SS-soluble and insoluble fractions for yrs 1 and 2. However, the latter were only detected in TCA-soluble fractions. These data suggest that needle proteins were not effectively precipitated and needles contained free pentoses or non-precipitable RNA (orcinol-reactive).

• OSTI ID: 5561112
• Report Number(s): CONF-9107184-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (United States), Vol. 96:1; Conference: Annual meeting of the American Society of Plant Physiology, Albuquerque, NM (United States), 28 Jul - 1 Aug 1991; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
ACID RAIN
ENVIRONMENTAL IMPACTS
OZONE
TOXICITY
COMPARATIVE EVALUATIONS
LEAVES
NECROSIS
PINES
PROTEINS
SAMPLE PREPARATION
ATMOSPHERIC PRECIPITATIONS
CONIFERS
EVALUATION
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PINOPHYTA
PLANTS
RAIN
TREES
551000* - Physiological Systems
540110