Polymerized soluble venom--human serum albumin
Extensive previous studies have demonstrated that attempts to produce polymers of Hymenoptera venoms for human immunotherapy resulted in insoluble precipitates that could be injected with safety but with very limited immunogenicity in allergic patients. We now report soluble polymers prepared by conjugating bee venom with human serum albumin with glutaraldehyde. The bee venom-albumin polymer (BVAP) preparation was fractionated on Sephacryl S-300 to have a molecular weight range higher than catalase. /sup 125/I-labeled bee venom phospholipase A was almost completely incorporated into BVAP. Rabbit antibody responses to bee venom and bee venom phospholipase A were induced by BVAP. Human antisera against bee venom were absorbed by BVAP. No new antigenic determinants on BVAP were present as evidenced by absorption of antisera against BVAP by bee venom and albumin. BVAP has potential immunotherapeutic value in patients with anaphylactic sensitivity to bee venom.
- Research Organization:
- Northwestern Univ. Medical School, Chicago, IL
- OSTI ID:
- 5549273
- Journal Information:
- J. Allergy Clin. Immunol.; (United States), Vol. 75:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNE SERUMS
SYNTHESIS
IMMUNOTHERAPY
VENOMS
LIQUID COLUMN CHROMATOGRAPHY
ALBUMINS
ANAPHYLAXIS
ANTIBODIES
BEES
IODINE 125
PHOSPHATASES
POLYMERS
SOLUBILITY
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
HYDROLASES
HYMENOPTERA
INSECTS
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE ISOTOPES
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
THERAPY
551001* - Physiological Systems- Tracer Techniques