Polymerized soluble venom--human serum albumin
Abstract
Extensive previous studies have demonstrated that attempts to produce polymers of Hymenoptera venoms for human immunotherapy resulted in insoluble precipitates that could be injected with safety but with very limited immunogenicity in allergic patients. We now report soluble polymers prepared by conjugating bee venom with human serum albumin with glutaraldehyde. The bee venom-albumin polymer (BVAP) preparation was fractionated on Sephacryl S-300 to have a molecular weight range higher than catalase. /sup 125/I-labeled bee venom phospholipase A was almost completely incorporated into BVAP. Rabbit antibody responses to bee venom and bee venom phospholipase A were induced by BVAP. Human antisera against bee venom were absorbed by BVAP. No new antigenic determinants on BVAP were present as evidenced by absorption of antisera against BVAP by bee venom and albumin. BVAP has potential immunotherapeutic value in patients with anaphylactic sensitivity to bee venom.
- Authors:
- Publication Date:
- Research Org.:
- Northwestern Univ. Medical School, Chicago, IL
- OSTI Identifier:
- 5549273
- Resource Type:
- Journal Article
- Journal Name:
- J. Allergy Clin. Immunol.; (United States)
- Additional Journal Information:
- Journal Volume: 75:3
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; IMMUNE SERUMS; SYNTHESIS; IMMUNOTHERAPY; VENOMS; LIQUID COLUMN CHROMATOGRAPHY; ALBUMINS; ANAPHYLAXIS; ANTIBODIES; BEES; IODINE 125; PHOSPHATASES; POLYMERS; SOLUBILITY; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; ESTERASES; HYDROLASES; HYMENOPTERA; INSECTS; INTERMEDIATE MASS NUCLEI; INVERTEBRATES; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; SEPARATION PROCESSES; THERAPY; 551001* - Physiological Systems- Tracer Techniques
Citation Formats
Patterson, R, Suszko, I M, and Grammer, L C. Polymerized soluble venom--human serum albumin. United States: N. p., 1985.
Web. doi:10.1016/0091-6749(85)90076-4.
Patterson, R, Suszko, I M, & Grammer, L C. Polymerized soluble venom--human serum albumin. United States. https://doi.org/10.1016/0091-6749(85)90076-4
Patterson, R, Suszko, I M, and Grammer, L C. 1985.
"Polymerized soluble venom--human serum albumin". United States. https://doi.org/10.1016/0091-6749(85)90076-4.
@article{osti_5549273,
title = {Polymerized soluble venom--human serum albumin},
author = {Patterson, R and Suszko, I M and Grammer, L C},
abstractNote = {Extensive previous studies have demonstrated that attempts to produce polymers of Hymenoptera venoms for human immunotherapy resulted in insoluble precipitates that could be injected with safety but with very limited immunogenicity in allergic patients. We now report soluble polymers prepared by conjugating bee venom with human serum albumin with glutaraldehyde. The bee venom-albumin polymer (BVAP) preparation was fractionated on Sephacryl S-300 to have a molecular weight range higher than catalase. /sup 125/I-labeled bee venom phospholipase A was almost completely incorporated into BVAP. Rabbit antibody responses to bee venom and bee venom phospholipase A were induced by BVAP. Human antisera against bee venom were absorbed by BVAP. No new antigenic determinants on BVAP were present as evidenced by absorption of antisera against BVAP by bee venom and albumin. BVAP has potential immunotherapeutic value in patients with anaphylactic sensitivity to bee venom.},
doi = {10.1016/0091-6749(85)90076-4},
url = {https://www.osti.gov/biblio/5549273},
journal = {J. Allergy Clin. Immunol.; (United States)},
number = ,
volume = 75:3,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 1985},
month = {Fri Mar 01 00:00:00 EST 1985}
}