Replica plating method for estimating phenanthrene-utilizing and phenanthrene-cometabolizing microorganisms

Journal Article · Tue Feb 01 00:00:00 EST 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5533690

Shiaris, M P ^[1]; Cooney, J J

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1. Chesapeake Biological Lab., Solomons, MD

A replica plating method was developed for detecting and enumerating phenanthrene-degrading microorganisms. The method is designed to discriminate between aquatic organisms that utilize phenanthrene as the sole carbon and energy source and organisms that cometabolize phenanthrene. The method was used to demonstrate that phenanthrene utilizers and phenanthrene cometabolizers coexist in estuarine sediments.

• OSTI ID: 5533690
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 45:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MICROORGANISMS
CULTIVATION TECHNIQUES
PHENANTHRENE
BIODEGRADATION
ESTUARIES
MEASURING METHODS
METABOLISM
SEDIMENTS
SEPARATION PROCESSES
AROMATICS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
ORGANIC COMPOUNDS
SURFACE WATERS
550700* - Microbiology