Tissue to plasma capillary permeability of sup 131 I-albumin in the perfused rabbit ear

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Microvascular Research; (United States)

DOI:https://doi.org/10.1016/0026-2862(91)90016-5· OSTI ID:5509665

Bent-Hansen, L; Svendsen, J H ^[1]

---

1. Department of Medicine B, Rigshospitalet, University of Copenhagen (Denmark)

The tissue to plasma transfer of {sup 131}I-albumin was recorded in perfused rabbit ears (n = 6) following equilibration for 24 hr. 125I-fibrinogen served as the plasma marker, and was introduced intravenously 15 min before clamping. The ears were rollerpump perfused with isotonic diluted plasma at a constant rate of (mean {plus minus} SD) 5.1 {plus minus} 1.5 ml (min.100 g)-1. The mean extravascular albumin distribution volume was 12.4 {plus minus} 1.1 ml.100 g-1, and the fibrinogen volume (plasma volume in tissue) was 3.1 {plus minus} 0.4 ml.100 g-1 as determined from biopsies of the contralateral ear. The initial transfer of albumin was marked, and occurred at rates corresponding to a unidirectional clearance (Cl(0)) of 0.068 {plus minus} 0.012 ml (min.100 g)-1. However, with a reduction of mean interstitial albumin tracer content of no more than 4%, net transport decreased to reach slowly declining levels 5 to 10 times lower within 10 min of continued perfusion. The decrease was considered due to rapid exhaustion of a small interstitial pool of tracer immediately adjacent to the exchange vessel membrane, followed by an increasingly retarded outwash from more distant areas. The results suggest a bimodal structural resistance to albumin movement: a relatively low resistance in the capillary membrane, and a considerable restriction to albumin transport located within the interstitial space.

• OSTI ID: 5509665
• Journal Information: Microvascular Research; (United States), Vol. 41:2; ISSN 0026-2862
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
MEMBRANE TRANSPORT
CAPILLARIES
PERMEABILITY
AUDITORY ORGANS
BIOLOGICAL MARKERS
BIOPSY
FIBRINOGEN
IODINE 125
IODINE 131
PERFUSED TISSUES
RABBITS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
SENSE ORGANS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques