Synaptic localization of. kappa. opioid receptors in guinea pig neostriatum

Journal Article · Wed Jan 15 00:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.2.564· OSTI ID:5488625

Jomary, C; Beaudet, A ^[1]; Gairin, J E ^[2]

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1. McGill Univ., Montreal, Quebec (Canada)
2. Centre National de la Recherche Scientifique, Toulouse (France)

Distribution of {kappa} opioid receptors was examined by EM radioautography in sections of guinea pig neostriatum with the selective {sup 125}I-labeled dynorphin analog (D-Pro{sup 10})dynorphin-(1-11). Most specifically labeled binding sites were found by probability circle analysis to be associated with neuronal membrane appositions. Because of limitations in resolution of the method, the radioactive sources could not be ascribed directly to either one of the apposed plasma membranes. Nevertheless, three lines of evidence favored a predominant association of ligand with dendrites of intrinsic striatal neurons: (1) the high frequency with which labeled interfaces implicated a dendrite, (2) the enrichment of dendrodendritic interfaces, and (3) the occurrence of dendritic profiles labeled at several contact points along their plasma membranes. A small proportion of labeled sites was associated with axo-axonic interfaces, which may subserve the {kappa} opioid-induced regulation of presynaptic dopamine and acetylcholine release documented in guinea pig neostriatum. These results support the hypothesis that in mammalian brain {kappa} opioid receptors are conformationally and functionally distinct from {mu} and {delta} types.

• OSTI ID: 5488625
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:2; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
NARCOTICS
CHEMORECEPTORS
ACETYLCHOLINE
CELL MEMBRANES
DOPAMINE
GUINEA PIGS
IODINE 125
NERVE CELLS
ULTRASTRUCTURAL CHANGES
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANES
MORPHOLOGICAL CHANGES
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PHENOLS
POLYPHENOLS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques