Reversible cAMP-induced translocation of cytoskeleton-associated 300- to 350-kDa proteins from nucleus to cytoplasm

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90469-7· OSTI ID:5451010

Nakayama, Tokiko; Nishizawa, Kimiko; Sato, Chicako ^[1]

---

1. Aichi Cancer Center Research Institute, Nagoya (Japan)

The authors previously reported that treatment of SV-3Y1 cells in an exponential growth state with db-cAMP plus theophylline induced reversible disappearance of nuclear dots stained by monoclonal anti-microtubule-associated protein (MAP)-1 antibody. In the present study, the authors examined the relation between the intracellular localization and phosphorylation of 300- to 350-kDa proteins that are intracellular antigens for our anti-Map-1 and -2 antibodies. Treatment with db-cAMP plus theophylline was found to result in a reversible decrease in immunofluorescent staining of the nucleus with polyclonal MAP-1 or -2 antibody, and a reversible increase in that of the cytoplasm. Simultaneous treatment with colchicine, colcemid, putrescine, or {alpha}-naphthyl phosphate in the presence of db-cAMP plus theophylline almost prevented this effect of db-cAMP plus theophylline. They examined the cytoplasmic and nuclear fractions by immunoperoxidase staining, immunoprecipitation, and {sup 125}I-protein A with anti-MAP-1 and -2 antibodies. The present research indicated that treatment with db-cAMP plus theophylline resulted in the reversible translocation of 300- to 350-kDa proteins from the nucleus to the cytoplasm accompanied by the dephosphorylation of these proteins.

• OSTI ID: 5451010
• Journal Information: Experimental Cell Research; (United States), Vol. 177:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
PHOSPHORYLATION
PROTEINS
TRANSLOCATION
AMP
CELL MEMBRANES
IODINE 125
MICROTUBULES
MONOCLONAL ANTIBODIES
ONCOGENIC TRANSFORMATIONS
THEOPHYLLINE
VIRUSES
ANTIBODIES
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
MEMBRANES
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
PURINES
RADIOISOTOPES
VASODILATORS
XANTHINES
550301* - Cytology- Tracer Techniques