Endotoxin (Etx) induced lung protein leak and loss of endothelial dependent relaxation
Endotoxin injures pulmonary vascular endothelium as assessed by histology and increased protein permeability. We hypothesized that another endothelial function might also be impaired by endotoxin. Lung protein leak was measured in rats given S. enteriditis Etx ip (4 mg/kg) with extravascular (EV) protein accumulation derived from total lung radiolabelled protein (/sup 125/I albumin) corrected for iv protein using /sup 51/Cr-RBC tag. Etx treated rats failed to show increased EV protein at 6 h after Etx but developed increased EV protein by 24 h. Endothelial dependent relaxation was assessed in rats using isolated pulmonary artery rings preconstricted with norepinephrine (10/sup -7/M). Endothelial dependent relaxation was induced by acetylcholine (10/sup -6/M, ACH) and calcium ionophore A23187 (10/sup -6/M,A23) and compared to endothelial independent relaxation with nitroprusside (10/sup -7/M, NP). Endothelial dependent relaxation was impaired only at 24 h. In conclusion, Etx induced a delayed pulmonary vascular endothelial injury in the rat as manifest by increased protein leak paralleled by diminished endothelial dependent relaxation.
- Research Organization:
- Univ. of Colorado Health Science Center, Denver
- OSTI ID:
- 5447501
- Report Number(s):
- CONF-8604222-; TRN: 86-028563
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ENDOTHELIUM
PERMEABILITY
ENDOTOXINS
BIOLOGICAL EFFECTS
LUNGS
INJURIES
ACETYLCHOLINE
ALBUMINS
CALCIUM
CHROMIUM 51
IODINE 125
NORADRENALINE
PROTEINS
RATS
TRACER TECHNIQUES
ADRENAL HORMONES
ALKALINE EARTH METALS
AMINES
AMMONIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
ANTIGENS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ESTERS
EVEN-ODD NUCLEI
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METALS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
TISSUES
TOXIC MATERIALS
TOXINS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550901 - Pathology- Tracer Techniques