Role of nitrite in the induction of nitrate reductase activity in barley leaves

Conference · Tue Apr 01 00:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5431489

Aslam, M; Huffaker, R C

High levels of nitrate reductase activity (NRA) were induced in detached leaves of 8-day-old barley (Hordeum vulgare L.) seedlings when supplied with NO/sub 2//sup -/ in the induction solutions. At similar N flux, the level of the enzyme activity induced by NO/sub 2//sup -/ was about one-half of that induced by NO/sub 3//sup -/. Significant levels of NO/sub 3//sup -/ accumulated in NO/sub 2//sup -/-fed leaves. Traces of NO/sub 3//sup -/ (0.6%) were detected in solutions of reagent grade KNO/sub 2/. However, the amount of NO/sub 3//sup -/ absorbed from the NO/sub 2//sup -/ solutions was only one-tenth of that accumulated in the leaves during the induction period, showing the actual conversion of NO/sub 2//sup -/ to NO/sub 3//sup -/ within the leaf. When the NO/sub 3//sup -/ concentrations in the NO/sub 2//sup -/-fed leaves were plotted against NRA, a highly positive correlation was obtained. The results suggest that NO/sub 2//sup -/ induces NRA indirectly after being oxidized to NO/sub 3//sup -/ within the leaf.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5431489
• Journal Information: Plant Physiol.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
NITRATES
BIOCHEMICAL REACTION KINETICS
NITRITES
BIOLOGICAL EFFECTS
OXIDOREDUCTASES
ENZYME INDUCTION
BARLEY
LEAVES
CEREALS
ENZYMES
GENE REGULATION
GRASS
KINETICS
NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
REACTION KINETICS
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)
550200 - Biochemistry