Helicase properties of the Escherichia coli UvrAb protein complex

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.11.3638· OSTI ID:5430992

Oh, E Y; Grossman, L

The Escherichia coli UvrA protein has an associated ATPase activity with a turnover number affected by the presence of UvrB protein as well as by DNA. Specifically, the structure of DNA significantly influences the turnover rate of the UvrAB ATPase activity. Double-stranded DNA maximally activates the turnover rate 10-fold whereas single-stranded DNA maximally activates the turnover rate 20-fold, suggesting that the mode of interaction of UvrAB protein with different DNAs is distinctive. We have previously shown that the UvrAB protein complex, driven by the binding energy of ATP, can locally unwind supercoiled DNA. The nature of the DNA unwinding activity and single-stranded DNA activation of ATPase activity suggest potential helicase activity. In the presence of a number of helicase substrates, the UvrAB complex, indeed, manifests a strand-displacement activity-unwinding short duplexes and D-loop DNA, thereby generating component DNA structures. The energy for the activity is derived from ATP or dATP hydrolysis. Unlike the E. coli DnaB, the UvrAB helicase is sensitive to UV-induced photoproducts.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 5430992
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:12
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
DNA HELICASES
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
ESCHERICHIA COLI
BIOLOGICAL RADIATION EFFECTS
DNA REPAIR
ATP-ASE
DNA
FAR ULTRAVIOLET RADIATION
NEAR ULTRAVIOLET RADIATION
PHOSPHORUS 32
PROTEINS
ACID ANHYDRASES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
560130* - Radiation Effects on Microorganisms
550601 - Medicine- Unsealed Radionuclides in Diagnostics