Presynaptic localization of histamine H3-receptors in rat brain

Journal Article · Fri Jun 28 00:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)90624-G· OSTI ID:5410338

Fujimoto, K; Mizuguchi, H; Fukui, H; Wada, H ^[1]

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1. Osaka Univ. (Japan)

The localization of histamine H3-receptors in subcellular fractions from the rat brain was examined in a (3H) (R) alpha-methylhistamine binding assay and compared with those of histamine H1- and adrenaline alpha 1- and alpha 2-receptors. Major (3H)(R) alpha-methylhistamine binding sites with increased specific activities ((3H)ligand binding vs. protein amount) were recovered from the P2 fraction by differential centrifugation. Minor (3H)(R)alpha-methylhistamine binding sites with increased specific activities were also detected in the P3 fraction. Further subfractionation of the P2 fraction by discontinuous sucrose density gradient centrifugation showed major recoveries of (3H)(R)alpha-methylhistamine binding in myelin (MYE) and synaptic plasma membrane (SPM) fractions. A further increase in specific activity was observed in the MYE fraction, but the SPM fraction showed no significant increase in specific activity. Adrenaline alpha 2-receptors, the pre-synaptic autoreceptors, in a (3H) yohimbine binding assay showed distribution patterns similar to histamine H3-receptors. On the other hand, post-synaptic histamine H1- and adrenaline alpha 1-receptors were closely localized and distributed mainly in the SPM fraction with increased specific activity. Only a negligible amount was recovered in the MYE fraction, unlike the histamine H3- and adrenaline alpha 2-receptors.

• OSTI ID: 5410338
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Vol. 177:3; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADRENALINE
RECEPTORS
HISTAMINE
BIOLOGICAL LOCALIZATION
BIOCHEMICAL REACTION KINETICS
BRAIN
CELL MEMBRANES
COMPARATIVE EVALUATIONS
LIGANDS
RADIOASSAY
RATS
SUBCELLULAR DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
ADRENAL HORMONES
AMINES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRIFUGATION
DISTRIBUTION
DRUGS
EVALUATION
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques