Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome
The coding of two rare lipoproteins by two genes, rlpA and rlpB, located in the leuS-dacA region (15 min) on the Escherichia coli chromosome was demonstrated by expression of subcloned genes in a maxicell system. The formation of these two proteins was inhibited by globomycin, which is an inhibitor of the signal peptidase for the known lipoproteins of E. coli. In each case, this inhibition was accompanied by formation of a new protein, which showed a slightly lower mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and which we suppose to be a prolipoprotein with an N-terminal signal peptide sequence similar to those of the bacterial major lipoproteins and lysis proteins of some bacteriocins. The incorporation of /sup 3/H-labeled palmitate and glycerol into the two lipoproteins was also observed. Sequencing of DNA showed that the two lipoprotein genes contained sequences that could code for signal peptide sequences of 17 amino acids (rlpA lipoprotein) and 18 amino acids (rlpB lipoprotein). The deduced sequences of the mature peptides consisted of 345 amino acids (M/sub r/ 35,615, rlpA lipoprotein) and 175 amino acids (M/sub r/ 19,445, rlpB lipoprotein), with an N-terminal cysteine to which thioglyceride and N-fatty acyl residues may be attached. These two lioproteins may be important in duplication of the cells.
- Research Organization:
- Univ. of Tokyo, Japan
- OSTI ID:
- 5409363
- Journal Information:
- J. Bacteriol.; (United States), Vol. 169:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESCHERICHIA COLI
MOLECULAR BIOLOGY
LIPOPROTEINS
DNA SEQUENCING
GENETIC MAPPING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BACTERIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAPPING
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550401* - Genetics- Tracer Techniques