Posttranscriptional regulation of cellular gene expression by the c-myc oncogene
- Princeton Univ., NJ (USA). Dept. of Biology
The c-myc oncogene has been implicated in the development of many different cancers, yet the mechanism by which the c-myc protein alters cellular growth control has proven elusive. The authors used a cDNA hybridization difference assay to isolate two genes, mr1 and mr2, that were constitutively expressed (i.e., deregulated) in rodent fibroblast cell lines immortalized by transfection of a viral promoter-linked c-myc gene. Both cDNAs were serum inducible in quiescent G/sub o/ fibroblasts, suggesting that they are functionally related to cellular proliferative processes. Although there were significant differences in cytoplasmic mRNA levels between myc-immortalized and control cells, the rates of transcription and mRNA turnover of both genes were similar, suggesting that c-myc regulates mr1 and mr2 expression by some nuclear posttranscriptional mechanism. Their results provide evidence that c-myc can rapidly modulate cellular gene expression and suggest that c-myc may function in gene regulation at the level of RNA export, splicing, or nuclear RNA turnover.
- OSTI ID:
- 5407181
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 9:1; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOMAS
GENE REGULATION
FIBROBLASTS
CELL CULTURES
ONCOGENES
MOLECULAR BIOLOGY
BIOCHEMISTRY
CELL DIFFERENTIATION
CELL DIVISION
DNA-CLONING
GENE AMPLIFICATION
GENETIC ENGINEERING
MAMMARY GLANDS
MESSENGER-RNA
ONCOGENIC VIRUSES
PHENOTYPE
POST-TRANSLATION MODIFICATION
RATS
TRANSCRIPTION
ANIMAL CELLS
ANIMALS
BODY
CHEMISTRY
CLONING
CONNECTIVE TISSUE CELLS
DISEASES
DNA HYBRIDIZATION
GENES
GLANDS
HYBRIDIZATION
MAMMALS
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
RNA
RODENTS
SOMATIC CELLS
VERTEBRATES
VIRUSES
550200* - Biochemistry
550400 - Genetics