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Title: Posttranscriptional regulation of cellular gene expression by the c-myc oncogene

The c-myc oncogene has been implicated in the development of many different cancers, yet the mechanism by which the c-myc protein alters cellular growth control has proven elusive. The authors used a cDNA hybridization difference assay to isolate two genes, mr1 and mr2, that were constitutively expressed (i.e., deregulated) in rodent fibroblast cell lines immortalized by transfection of a viral promoter-linked c-myc gene. Both cDNAs were serum inducible in quiescent G/sub o/ fibroblasts, suggesting that they are functionally related to cellular proliferative processes. Although there were significant differences in cytoplasmic mRNA levels between myc-immortalized and control cells, the rates of transcription and mRNA turnover of both genes were similar, suggesting that c-myc regulates mr1 and mr2 expression by some nuclear posttranscriptional mechanism. Their results provide evidence that c-myc can rapidly modulate cellular gene expression and suggest that c-myc may function in gene regulation at the level of RNA export, splicing, or nuclear RNA turnover.
Authors:
;  [1]
  1. (Princeton Univ., NJ (USA). Dept. of Biology)
Publication Date:
OSTI Identifier:
5407181
Resource Type:
Journal Article
Resource Relation:
Journal Name: Molecular and Cellular Biology; (USA); Journal Volume: 9:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARCINOMAS; GENE REGULATION; FIBROBLASTS; CELL CULTURES; ONCOGENES; MOLECULAR BIOLOGY; BIOCHEMISTRY; CELL DIFFERENTIATION; CELL DIVISION; DNA-CLONING; GENE AMPLIFICATION; GENETIC ENGINEERING; MAMMARY GLANDS; MESSENGER-RNA; ONCOGENIC VIRUSES; PHENOTYPE; POST-TRANSLATION MODIFICATION; RATS; TRANSCRIPTION; ANIMAL CELLS; ANIMALS; BODY; CHEMISTRY; CLONING; CONNECTIVE TISSUE CELLS; DISEASES; DNA HYBRIDIZATION; GENES; GLANDS; HYBRIDIZATION; MAMMALS; MICROORGANISMS; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PARASITES; RNA; RODENTS; SOMATIC CELLS; VERTEBRATES; VIRUSES 550200* -- Biochemistry; 550400 -- Genetics