Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells

Journal Article · Tue May 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.10.3738· OSTI ID:5389550

Bodine, D M; McDonagh, K T; Brandt, S J; Ney, P A; Agricola, B; Byrne, E; Nienhuis, A W ^[1]

---

1. National Institutes of Health, Bethesda, MD (USA)

Retroviral-mediated gene transfer into primitive hematopoietic cells has been difficult to achieve in large-animal models. The authors have developed an amphotropic producer clone that generates >10{sup 10} recombinant retroviral particles (colony-forming units) per ml of culture medium. Autologous rhesus monkey bone marrow cells were cocultured with either high or low titer producer clones for 4-6 days and reinfused into sublethally irradiated animals. The proviral genome was detected in blood and bone-marrow cells from all three animals reconstituted with cells cocultured with the high-titer producer cells. In contrast, three animals reconstituted with bone marrow cocultured with the low-titer producer clone exhibited no evidence of gene transfer.

• OSTI ID: 5389550
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:10; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
GENE RECOMBINATION
GROWTH FACTORS
BIOSYNTHESIS
MONKEYS
SUBLETHAL IRRADIATION
GENE AMPLIFICATION
STEM CELLS
VIRUSES
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
IRRADIATION
MAMMALS
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
PROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals