Fluoride (F) is not taken up by Salmonella typhimurium TA98 (TA98), used for Ames mutagenicity test, unless aluminum (Al) is present

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5374053

Ahn, H W; Jeffery, E H ^[1]

---

1. Univ. of Illinois, Urbana (United States)

TA98 is commonly used for the Ames test to detect frame-shift mutations. Ames test results for fluoride mutagenicity are controversial. Since Al and F, both present in finished drinking water, form a stable complex, the authors determined the effect of Al on F uptake by TA98 to evaluate any Al effect on Ames test results for F. A known number of TA98 in 0.1M potassium phosphate buffer was incubated with various concentrations of Al, F or aluminum fluoride for 1 hr at 37C in a shaking incubator. The cells were washed twice in potassium phosphate buffer and then resuspended in water. After breaking the cells open by sonication, the resulting supernatant was analyzed for Al by graphite furnace atomic absorption and for F using a F ion-selective electrode. Al accumulated in a concentration-dependent manner to 4.5 ppm then decreased rapidly as the Al was increased to 9 ppm. Intracellular F was below the limit of detection even when the medium contained 590 ppm F. However, F was taken up from media containing greater than 200 ppm F, providing that aluminum was also present. The authors conclude that the Ames test, using TA98, is not suitable for the detection of F mutagenicity.

• OSTI ID: 5374053
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
BIOLOGICAL EFFECTS
FLUORIDES
UPTAKE
ABSORPTION SPECTRA
DOSE-RESPONSE RELATIONSHIPS
METABOLIC ACTIVATION
MUTAGEN SCREENING
SALMONELLA TYPHIMURIUM
BACTERIA
ELEMENTS
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
METALS
MICROORGANISMS
SALMONELLA
SCREENING
SPECTRA
560300* - Chemicals Metabolism & Toxicology