Glucocorticoid suppression of human lymphocyte DNA synthesis. Influence of phytohemagglutinin concentration
Glucocorticoids have been shown to suppress lectin-stimulated lymphocyte DNA synthesis in some studies, whereas in other studies, the hormones have had little effect. We have found that the position on the PHA dose-response curve that is studied is the most important determinant of whether cortisol inhibits /sup 3/H-thymidine incorporation into lymphocyte DNA. The proportion of monocytes in culture also influenced the cortisol effect, but it was quantitatively less important than PHA concentration. Cortisol (5 nM to 100 ..mu..M) had little effect on blastogenesis or thymidine incorporation into DNA in cultures that contained both a high concentration (14% +- 2 (S.E.)) of monocytes and a concentration of PHA (0.6 to 1.2 ..mu..g/ml) that produced maximal stimulation of mitogenesis. When monocytes were reduced from 14 to 1.4%, cortisol (5 ..mu..M) caused a 30% reduction in thymidine incorporation in cultures stimulated by 0.6 to 1.2 ..mu..g/ml PHA. Much greater cortisol suppression of thymidine incorporation occurred if the concentration of PHA was reduced. For example, reduction of the PHA concentration from 1.2 to 0.075 ..mu..g/ml resulted in an increase in suppression by 5 ..mu..M cortisol from 5 to 90% even in the presence of 14% monocytes. These data indicate that the suppressive effects of glucocorticoids on blastogenesis and thymidine incorporation in vitro depend principally on the concentration of PHA used to stimulate blastogenesis and secondarily on the proportion of monocytes in the culture system.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 5373329
- Journal Information:
- J. Lab. Clin. Med.; (United States), Vol. 95:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOSYNTHESIS
GLUCOCORTICOIDS
BIOLOGICAL EFFECTS
PHYTOHEMAGGLUTININ
CELL CULTURES
HYDROCORTISONE
INHIBITION
LYMPHOCYTES
MAN
MONOCYTES
STEM CELLS
THYMIDINE
TRITIUM COMPOUNDS
ADRENAL HORMONES
AGGLUTININS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PREGNANES
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550300 - Cytology