Phenol induced acute cutaneous inflammation (AI) in mice: Diminished response in mast cell-deficient (W/W sup v ) mice and evidence of a role for tumor necrosis factor-alpha (TNF)

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5371534

Wershil, B K; Wang, Z S; Gordon, J R; Galli, S J ^[1]

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1. Beth Israel Hospital, Boston, MA (United States) Harvard Medical School, Boston, MA (United States)

AI can be induced by a variety of chemical agents. The authors examined AI in mast cell-deficient (WBB6F{sub 1}-W/W{sup v}) and congenic normal (WBB6F{sub 1}-+/+) mice; AI was induced by the epicutaneous application to the ear of phenol (2 mg), benzalkonium chloride (BC; 1 mg) and ethyl phenylpropiolate (EPP, 2 or 5 mg). Phenol induced significantly greater swelling in +/+ than in W/W{sup v} mice. No difference in swelling was seen in +/+ versus W/W{sup v} mice with BC or EEP. Phenol application induced significantly greater neutrophil infiltration in +/+ than in W/W{sup v} mice. Mast cells represent a rich source of TNF and TNF has been shown to participate in the neutrophil accumulation seen in mast cell-dependent, IgE-mediated cutaneous late phase reactions. The authors injected +/+ mice i.d. with 20 {mu}l of 1:100 dilution of a polyclonal rabbit anti-mouse TNF antiserum or 20 {mu}l of medium and then applied 2 mg phenol at the same sites. At 24 hrs, significantly less neutrophil accumulation was seen in the ear treated with anti-TNF antibodies than in the control ear. The authors conclude that mast cells may participate in phenol-induced AI, and that TNF contributes to this response.

• OSTI ID: 5371534
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MAST CELLS
BIOLOGICAL FUNCTIONS
PHENOL
TOXICITY
PROTEINS
ANTIBODIES
MICE
NECROSIS
NEOPLASMS
NEUTROPHILS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
HYDROXY COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHENOLS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology