Induction of nerve growth factor receptors on cultured human melanocytes
- Tufts Univ., Boston, MA (USA)
Normal differentiation and malignant transformation of human melanocytes involve a complex series of interactions during which both genetic and environmental factors play roles. At present, the regulation of these processes is poorly understood. The authors have induced the expression of nerve growth factor (NGF) receptors on cultured human melanocytes with phorbol 12-tetradecanoate 13-acetate and have correlated this event with the appearance of a more differentiated, dendritic morphology. Criteria for NGF receptor expression included protein accumulation and cell-surface immunofluorescent staining with a monoclonal antibody directed against the human receptor and induction of the messenger RNA species as determined by blot-hybridization studies. The presence of the receptor could also be induced by UV irradiation or growth factor deprivation. The NGF receptor is inducible in cultured human melanocytes, and they suggest that NGF may modulate the behavior of this neural crest-derived cell in the skin.
- OSTI ID:
- 5365086
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:14; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROWTH FACTORS
RECEPTORS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ANIMAL CELLS
CELL CULTURES
EXTREME ULTRAVIOLET RADIATION
FLUORESCENCE
MELANIN
SKIN
BODY
CARCINOGENS
ELECTROMAGNETIC RADIATION
ESTERS
HYDROXY COMPOUNDS
LUMINESCENCE
MEMBRANE PROTEINS
MITOGENS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
PROTEINS
RADIATIONS
ULTRAVIOLET RADIATION
550201* - Biochemistry- Tracer Techniques