Transient increase in phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol trisphosphate during activation of human neutrophils

Journal Article · Fri Sep 15 00:00:00 EDT 1989 · Journal of Biological Chemistry; (USA)

OSTI ID:5364696

Traynor-Kaplan, A E; Thompson, B L; Harris, A L; Taylor, P; Omann, G M; Sklar, L A ^[1]

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1. Research Institute of Scripps Clinic, La Jolla, CA (USA)

We recently showed that phosphatidylinositol trisphosphate (PIP3) was present in a unique lipid fraction generated in neutrophils during activation. Here, we demonstrate that the band containing this fraction isolated from thin layer chromatography consists primarily of PIP3 and that only small amounts of radiolabeled PIP3 exist prior to activation. In addition, high performance liquid chromatography of deacylated phospholipids from stimulated cells reveals an increase in a fraction eluting ahead of glycerophosphoinositol 4,5-P2. After removal of the glycerol we found that it coeluted with inositol 1,3,4-P3 when resubjected to high performance liquid chromatography. Thus, we have detected a second, novel form of phosphatidylinositol bisphosphate in activated neutrophils, PI-(3,4)P2. The elevation of PIP3 through the formyl peptide receptor is blocked by pretreatment with pertussis toxin, implicating mediation of the increase in PIP3 by a guanosine triphosphate-binding (G) protein. The rise in PIP3 is not secondary to calcium elevation. Buffering the rise in intracellular calcium did not diminish the increase in PIP3. The elevation of PIP3 appears to occur during activation with physiological agonists, its level varying with the degree of activation. Leukotriene B4, which elicits many of the same responses as stimulation of the formyl peptide receptor but with minimal oxidant production, stimulates a much attenuated rise in PIP3. Isoproterenol, which inhibits oxidant production also reduces the rise in PIP3. Hence formation of PI(3,4)P2 and PIP3 (presumed to be PI(3,4,5)P3) correlates closely with the early events of neutrophil activation.

• OSTI ID: 5364696
• Journal Information: Journal of Biological Chemistry; (USA), Vol. 264:26; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NEUTROPHILS
CHEMICAL COMPOSITION
PHOSPHOLIPIDS
BIOSYNTHESIS
AUTORADIOGRAPHY
CALCIUM
EGTA
IN VITRO
LIQUID COLUMN CHROMATOGRAPHY
PHOSPHATES
PHOSPHORUS ISOTOPES
THIN-LAYER CHROMATOGRAPHY
ALCOHOLS
ALKALINE EARTH METALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMATOGRAPHY
ELEMENTS
ESTERS
GLYCOLS
HYDROXY COMPOUNDS
ISOTOPES
LEUKOCYTES
LIPIDS
MATERIALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques