Comparisons of three dry application autoradiographic techniques
- Virginia Polytechnic Institute and State Univ., Blacksburg (USA)
We compared three common dry application techniques applied for the first time to phytoplankton taking up water-soluble radioisotopically labeled substrates. Following incubation of live phytoplankton communities in oligotrophic, nitrogen-limited Mountain Lake, Virginia, with Carbon-14 labeled methylamine-hydrochloride, an ammomium analog, we concentrated cells on 0.8 um pore size Millipore filters, then preserved filters in liquid nitrogen, and freeze-dried. Differences in the techniques are application of stripping film, preparation of freshly prepared film by dipping loops into liquid emulsion, and dipping cover slips in liquid emulsion. Following dark-incubation and development, autoradiographs were evaluated microscopically for reproducibility, fine resolution of silver grains, background scatter, and eaes of technique.
- OSTI ID:
- 5352745
- Report Number(s):
- CONF-8708345-; CODEN: JPYLA; TRN: 90-000856
- Journal Information:
- Journal of Phycology; (USA), Vol. 23; Conference: Annual meeting of the Phycological Society of America, Columbus, OH (USA), 9-13 Aug 1987; ISSN 0022-3646
- Country of Publication:
- United States
- Language:
- English
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