Characterization of Mycobacterium paratuberculosis by gas-liquid and thin-layer chromatography and rapid demonstration of mycobactin dependence using radiometric methods
Thirty-six Mycobacterium paratuberculosis isolates of bovine, caprine, and ovine origins were evaluated by using gas-liquid chromatography (GLC), thin-layer chromatography (TLC), and BACTEC 7H12 Middlebrook TB medium in an effort to more rapidly differentiate this group of organisms from other mycobacteria. Bacterial suspensions (0.1 ml) were inoculated by syringe into 7H12 broth containing 2 micrograms of mycobactin P per ml and control broth without mycobactin P. Cultures were incubated at 37/sup 0/C and read daily with a BACTEC Model 301. After 8 days of incubation, the growth index readings for the test broths containing mycobactin P were twice those of the control broths without mycobactin P. Sixty-five isolates of mycobacteria other than M. paratuberculosis were also examined. No difference was noted between the growth index readings of control and mycobactin-containing broths. Except for Mycobacterium avium-Mycobacterium intracellulare, TLC studies differentiated M. paratuberculosis from the other mycobacterial species tested. The GLC data reveal that all M. paratuberculosis isolates had a distinctive peak (14A) which was not found among M. avium-M. intracellulare complex organisms. These data indicate that 7H12 radiometric broth was able to rapidly demonstrate the mycobactin dependence of M. paratuberculosis and GLC and TLC procedures were capable of rapidly differentiating this organism from the other mycobacteria studied.
- Research Organization:
- Colorado State Univ., Fort Collins (USA)
- OSTI ID:
- 5351996
- Journal Information:
- J. Clin. Microbiol.; (United States), Vol. 25:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MYCOBACTERIUM
CLASSIFICATION
GROWTH
CULTURE MEDIA
GAS CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
OXAZOLES
RADIOMETRIC ANALYSIS
TAXONOMY
THIN-LAYER CHROMATOGRAPHY
AZOLES
BACTERIA
BIOLOGY
CHEMICAL ANALYSIS
CHROMATOGRAPHY
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
550701* - Microbiology- Tracer Techniques