Production of acetaldehyde by Zymomonas mobilis
Mutants of Zymomonas mobilis were selected for decreased alcohol dehydrogenase activity by using consecutively higher concentration of allyl alcohol. A mutant selected by using 100 mM allyl alcohol produced acetaldehyde at a level of 4.08 g/liter when the organism was grown in aerated batch cultures on a medium containing 4.0% (wt/wt) glucose. On the basis of the amount of glucose utilized, this level of acetaldehyde production represents nearly 40% of the maximum theoretical yield. Acetaldehyde produced during growth was continuously air stripped from the reactor. Acetaldehyde present in the exhaust stream was then trapped as the acetaldehyde-bisulfite addition product in an aqueous solution of sodium bisulfite and released by treatment with base. Acetaldehyde was found to inhibit growth of Z. mobilis at concentrations as low as 0.05% (wt/wt) acetaldehyde. An acetaldehyde-tolerant mutant of Z. mobilis was isolated after both mutagenesis with nitrosoguanidine and selection in the presence of vapor-phase acetaldehyde. The production of acetaldehyde has potential advantages over that of ethanol: lower energy requirements for production separation, efficient separation of product from dilute feed streams, continuous separation of product from the reactor, and a higher marketplace value.
- Research Organization:
- Cornell Univ., Ithaca, NY
- OSTI ID:
- 5338152
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 53:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ACETALDEHYDE
BIOSYNTHESIS
TOXICITY
GLUCOSE
BIODEGRADATION
ZYMOMONAS MOBILIS
ENZYME ACTIVITY
COMPARATIVE EVALUATIONS
ETHANOL
MUTAGENESIS
MUTANTS
NITROSO COMPOUNDS
OXIDOREDUCTASES
TOLERANCE
ALCOHOLS
ALDEHYDES
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SACCHARIDES
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
560300 - Chemicals Metabolism & Toxicology
550700 - Microbiology