Synthesis and evaluation of radioactive and fluorescent residualizing labels for identifying sites of plasma protein catabolism
Inulin and lactose were each coupled to tyramine by reductive amination with NaBH/sub 3/CN and the tyramine then labeled with /sup 125/I. Dilactitol-/sup 125/I-tyramine (DLT) and inulin-/sup 125/I-tyramine (InTn) were coupled by reductive amination and cyanuric chloride, respectively, to asialofetuin (ASF), fetuin and rat serum albumin (RSA). Attachment of either label had no effect on the circulating half-lives of the proteins. Radioactivity from labeled ASF was recovered in rat liver (> 90%) by 1 h post-injection and remained in liver with half-lives of 2 and 6 days, respectively, for the DLT and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn-labeled RSA were 5 and 6.5 days, respectively, again indicating that the larger glycoconjugate label residualized more efficiently in cells following protein degradation. (Lactitol)/sub 2/-N-CH/sub 2/-CH/sub 2/-NH-fluroescein (DLF) was also coupled to ASF by reductive amination and recovered quantitatively in liver at 1 h post-injection. Native ASF was an effective competitor for clearance of DLF-ASF from the circulation. Fluorescent degradation products were retained in liver with a half-life of 1.2 days. Residualizing fluorescent labels should be useful for identification and sorting of cells active in the degradation of plasma proteins.
- Research Organization:
- Univ. of South Carolina, Columbia
- OSTI ID:
- 5326472
- Report Number(s):
- CONF-8606151-; TRN: 86-031401
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification of fibroblasts as a major site of albumin catabolism in peripheral tissues
Sites of catabolism of murine monomeric IgA
Related Subjects
INULIN
AMINATION
LACTOSE
PROTEINS
CATABOLISM
LABELLING
TYRAMINE
ALBUMINS
BIOLOGICAL HALF-LIFE
BIOSYNTHESIS
BLOOD PLASMA
BLOOD-PLASMA CLEARANCE
EVALUATION
FLUORESCENCE
IODINE 125
LIVER
RATS
TRACER TECHNIQUES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBOHYDRATES
CHEMICAL REACTIONS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISACCHARIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MAMMALS
MATERIALS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYSACCHARIDES
RADIOISOTOPES
RODENTS
SACCHARIDES
SYMPATHOMIMETICS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques